Influenza A virus (H1N1) is an acute, highly contagious respiratory virus. The use of lactic acid bacteria (LAB) to deliver mucosal vaccines against influenza virus infection is a research hot spot. In this study, two recombinant Lactobacillus plantarum strains expressing hemagglutinin (HA) alone or coexpressing aCD11c-HA to target HA protein to dendritic cells (DCs) by fusion to an anti-CD11c

Probi Mage® bygger på mjölksyrabakterien Lactobacillus plantarum 299v (LP299V®). Denna bakterie är ett resultat av drygt tjugo års svensk forskning och är

2020-12-01 · To develop a more effective and safe oral vaccine against rabies, we constructed recombinant Lactobacillus plantarum NC8 carrying one or two copies of the G gene with a dendritic cell-targeting peptide (DCpep) fused at the C-terminal designated NC8-pSIP409-sRVG or NC8-pSIP409-dRVG, respectively. A high expression system in Escherichia coli BL21 (DE3) was constructed to produce and characterise the biochemical properties of the MurI from Lactobacillus plantarum NC8. In a 4-L-bioreactor cultivation, 3266 nkat(D-Glu)/mg(protein) of specific enzyme activity was produced. Lactobacillus plantarum is a versatile bacterium found in a variety of ecological niches, ranging from vegetable and plant fermentations to the human gastrointestinal tract. L. plantarum cells are rods with rounded ends, straight, generally 0.9–1.2 μm wide and 3–8 μm long, occurring singly, in pairs or in short chains (Figure 43.1). Lactobacillus plantarum NC8 Strain in the Infectious Bronchitis V irus V accination of Chickens Shaohua Guo 1, † , Junjie Peng 1, † , Yongle Xiao 1, † , Yanyan Liu 1 , W eiwei Hao 2 , Xin Y Bakterier: Lactobacillus rhamnosus GG och Bifidobacterium Lactis BB-12. En tablett ska innehålla 2 miljarder bakterier. (Säljs även som droppar).

(2004) L. plantarum NC8:pSIG308 Derivative of NC8; produces PLNC8IF constitutively from plasmid pSIG308. ErmR Constitutive (Bac+) We have recently shown that Lactobacillus plantarum NC8 was unable to produce bacteriocin when it was grown alone in broth cultures. However, cocultivation of L. plantarum NC8 with certain gram-positive bacteria in liquid cultures resulted in production of bacteriocin by this strain (A. Maldonado, J. L. 2020-12-10 · Axelsson, L. et al.

2020-12-01 · To develop a more effective and safe oral vaccine against rabies, we constructed recombinant Lactobacillus plantarum NC8 carrying one or two copies of the G gene with a dendritic cell-targeting peptide (DCpep) fused at the C-terminal designated NC8-pSIP409-sRVG or NC8-pSIP409-dRVG, respectively.

In this study, a recombinant Lactobacillus plantarum, designated as NC8-ChIL17B, was constructed to express the chicken IL-17B (ChIL-17B) gene. The recombinant ChIL17B (rChIL17B) protein was about 14 kDa and was anchored to the surface of NC8 cells. 5 Lactobacillus plantarum NC8, has been recently purified and genetically characterized. 6 Analysis of an 8.1-kb NC8 DNA region downstream of the PLNC8 operon revealed the 7 presence of at least four operons involved in bacteriocin production, showing high 8 homology to the plantaricin cluster in L. plantarum C11. However, we found a three- Lactobacillus plantarum NC8. Generally, this enzyme is not present in Lactobacillus spp.

uppsala Brudklänning plantarum nc8 sårade Lactobacillus. 2018 började SVT på nytt att reprisera avsnitt fler säsonger är planerade att visas. Kunde utöka sitt

L. plantarum NC8-competent cells were prepared as previously described . The L. plantarum NC8/Δalr strain was cultured in MRS medium with 200 μg/mL d-ala (Sigma, USA).

ABSTRACT Blanched and nonblanched potato rods (var. Beate) were fermented with Lactobacillus plantarum strain NC8 (109 colony-forming units [CFU]/mL) … Lactobacillus plantarum NC8 cultures which had been induced with Lactococcus lactis MG1363 or Pediococcus pentosaceus FBB63. This bacteriocin consists of two distinct peptides, named and, which were separated by C 2-C 18 reverse-phase chromatography and whose complementary action is necessary for full plantaricin NC8 Transformation of Lb. sakei Lb790, Lb. plantarum NC8 and Lb. casei CECT475 with plasmids pSIP409UAI, pSIP411UAI and pMGUAI yielded recombinant Lactobacillus spp.-derived strains which were further checked by bacteriocinogenicity tests, PCR and sequencing of the inserts. particular, Lactobacillus plantarum strains can target a series of pathogens, such as Helicobacter felis (10), Mycobacterium tuberculosis (11), and even tumor antigens (12). In addition, recombinant L. plantarum NC8 strains expressing the M2e or HA2 antigen constructed by Yang et al. have a protective effect after H9N2 subtype avian inﬂuenza Lactobacillus plantarum NC8 cells harbouring pGM4‐gus were induced at an O.D. 600 of ca 0·3 by addition of pheromone (IP‐673) to a final concentration of 50 ng ml −1.
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To accomplish this, a mucosal vaccine of a recombinant NC8 strain expressing HA and DCpep that was constructed in a previous study was employed.

Considering to the critical role of dendritic cells in stimulating host immune response, in this study, we constructed a novel CD11c-targeting L. plantarum strain with surface-displayed variable fragments of anti-CD11c, single-chain antibody (scFv-CD11c).
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Aug 22, 2014 Plantaricin C, plantaricin JK and plantaricin NC8, like nisin, appear to inhibit Supernatants of L. plantarum ZJ5 cultures were precipitated with

Maldonado, J. L. Ruiz-Barba, and R. Jiménez-Díaz, submitted for publication). Gene araA encoding the l-arabinose isomerase (l-AI) from Lactobacillus plantarum NC8 was cloned and expressed in Escherichia coli. It encodes a polypeptide of 474 residues having 55% identities with l- AIs from Bacillus stearothermophilus US100 and Thermus sp. IM6501.

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plantarum strains. NC8 has been used as a model strain in the development of genetic tools, such as conjugation (14), transformation (2), and expression vectors

Oxdc gene from Bacillus subtilis was polymerase chain reaction-ampliﬁed and Lactobacillus plantarum is a highly versatile lactic acid bacterium found in various ecological niches, such as fermented vegetable, meat, and dairy products and the gastrointestinal tract. We sequenced the genome of L. plantarum NC8, a naturally plasmid-free strain, which has been used as a model strain in many laboratories worldwide. Dendritic Cells Targeting Lactobacillus plantarum Strain NC8 with a Surface-Displayed Single-Chain Variable Fragment of CD11c Induce an Antigen-Specific Protective Cellular Immune Response Jing Liu , Guilian Yang , Haibin Huang , Chunwei Shi , Xing Gao , Wentao Yang , Zan Zhang , Yang Liu , Ke Xu , Jianzhong Wang , Yuanhuan Kang , Yanlong Jiang , Chunfeng Wang Lactobacillus plantarum subsp. plantarum NC8 genome sequencing project Lactobacillus plantarum subsp. plantarum NC8: Taxonomy navigation › Lactobacillus plantarum subsp.

Lactobacillus plantarum NC8 was used as host for aiiA (Bacillus thuringiensis 4A3 lactonase gene) using pSIP409 expression vector. pSIP409: aiiA construct was stably maintained in L. plantarum NC8.

particular, Lactobacillus plantarum strains can target a series of pathogens, such as Helicobacter felis (10), Mycobacterium tuberculosis (11), and even tumor antigens (12). In addition, recombinant L. plantarum NC8 strains expressing the M2e or HA2 antigen constructed by Yang et al. have a protective effect after H9N2 subtype avian inﬂuenza Lactobacillus plantarum NC8 cells harbouring pGM4‐gus were induced at an O.D. 600 of ca 0·3 by addition of pheromone (IP‐673) to a final concentration of 50 ng ml −1. β‐Glucuronidase activities were determined using a modified β‐galactosidase assay (Miller 1972) as described by Axelsson et al.. One of the most widespread Lactobacillus species used in food technology is Lactobacillus plantarum, which has a homofermentative metabolism, a high acid tolerance and is, like most LAB, a Generally Regarded As Safe (GRAS) organism. Experiments with deﬁned media have shown that Lact.

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